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| Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A.. |
| In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004 |
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| Travería,G.E.; Zumarraga,M.; Etchechoury,I.; Romano,M.I.; Cataldi,A.; Alvarado Pinedo,M.F.; Pavlik,I.; Pribylova,R.; Romero,J.R.. |
| We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Sheep; Typing; PCR; PCR-REA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300037 |
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